Microrheology with Optical Tweezers - Principles and Applications
by Manlio Tassieri (University of Glasgow, UK)
Hardback 312 pages 2016-09-30 Print ISBN: 9789814669184 eBook ISBN: 9789814669191 DOI: 10.4032/9789814669191
List price : $149.95
• Practical and user-friendly textbook that can be adopted for introductory undergraduate courses in physics, mathematics, chemistry, engineering, and materials science
• Demonstrates the physics underpinning the working principles of microrheology with optical tweezers
• Illustrated throughout with excellent figures and references accompanying each section
• Offers numerous interesting, well-illustrated, and recent examples from all the authors’ research work. The combined authorship further provides a unique multidisciplinary flavor needed for the teaching of microrheology of biological systems
Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to ?s. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)!
Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies.
The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the “nature of light,” followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.
|1||Chapter 1: General Introduction to Optical Tweezers and Their Applications|
|9||Chapter 2: The Nature of Light|
R. Mike L. Evans
|41||Chapter 3: Geometrical Optics|
|81||Chapter 4: Optical Forces|
Michael P. Lee and David B. Phillips
|103||Chapter 5: Optical Tweezers Configurations|
Graham M. Gibson
|137||Chatper 6: Introduction to Linear Rheology|
|155||Chapter 7: Statistical Mechanics and Diffusion Processes|
|193||Chapter 8: Most Popular Microrheology Techniques|
|219||Chapter 9: Microrheology with Optical Tweezers|
|259||Chapter 10: Optical Tweezers Outwith Microrheology|
Richard W. Bowman
Undergraduate and graduate students of physics, mathematics, chemistry, engineering, and materials science; researchers in biophysics
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